The antibody was originally tested on mda mb 361 cell lysate so this is a recommended positive control. Atf6 is a constitutively expressed endoplasmic reticulum er membrane anchored transcription factor. If using antibodies from cell signaling technology please see recommended dilution listed on the datasheet or product webpage and calculate the amount µg of igg antibody for negative control based on the cell signaling antibody concentration for a fair comparison.
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